Abstract: AimTo investigate the glycosidic constituents in the rhizomes of Alpinia officinarum Hance. MethodsThe isolation and purification of glycosides were done with column chromatography on macro porous resin, polyamides and Sephadex LH-20, whilst the structure elucidation was done by HRCI-MS and NMR (1D and 2D) methods. ResultsA glycosidic ester identified as 4′-hydroxy-2′-methoxyphenol-β-D-6-O-［4″-hydroxy-3″，5″-dimethoxy(benzoate)］-glucopyranoside (I), along with a known compound n-butyl-β-D-fructopyranoside (II), were isolated and characterized. ConclusionI was found to be a new compound, named as alpinoside A, whilst II was isolated from the genus Alpinia for the first time.