Abstract: Bioselective membrane electrode for L-ascorbic acid has been made with tissue slices. It is made from procine liver tissue slice covering an oxygen electrode. The influence of buffer mediator, pH, temperature, antiseptics, slice thickness, etc. on the response characteristics of the electrode was examined. In the presence of copper ions, a linear relationship between dynamic response of the electrode and the logarithm of concentration of L-ascorbie acid was observed for the range of 21.14 μg/ml to 352.3 μg/ml, with a correlation coefficient of 0.9996 and the Michaelis constant of 0.8 mmol/L. The electrode has been used to determine L-ascorbie acid in drugs, the analytical results were satisfactory.