徐晓虹, 郭丹, 杜红燕, 张维宁, 吴馥梅. 神经生长因子对小鼠突触体内Ca2+水平的调节作用J. 药学学报, 1997, 32(10): 730-734.
引用本文: 徐晓虹, 郭丹, 杜红燕, 张维宁, 吴馥梅. 神经生长因子对小鼠突触体内Ca2+水平的调节作用J. 药学学报, 1997, 32(10): 730-734.
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XH Xu, D Guo, HY Du, WN Zhang , FM Wu, . REGULATION OF NGF ON INTRASYNAPTOSOMAL FREE Ca2+i IN MOUSE BRAINJ. Acta Pharmaceutica Sinica, 1997, 32(10): 730-734.
Citation: XH Xu, D Guo, HY Du, WN Zhang , FM Wu, . REGULATION OF NGF ON INTRASYNAPTOSOMAL FREE Ca2+i IN MOUSE BRAINJ. Acta Pharmaceutica Sinica, 1997, 32(10): 730-734.
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神经生长因子对小鼠突触体内Ca2+水平的调节作用

REGULATION OF NGF ON INTRASYNAPTOSOMAL FREE Ca2+i IN MOUSE BRAIN

    摘要
  • 摘要: 观察了多次海马内微注射NGF对小鼠突触体内游离钙水平的影响,并在离体情况下观察NGF对EGTA和CaCl2分别造成突触体内低钙和高钙状态的调节作用。结果如下:(1)在体实验表明,一定剂量的NGF可显著降低老年小鼠海马突触体内游离钙水平(P<0.05);(2)离体实验表明,当突触体游离钙水平降低时,适当剂量的NGF具有升高游离钙水平的作用;而突触体内游离钙水平升高时,则NGF有降低游离钙水平的作用。提示NGF对游离钙水平的双向调节作用可能是NGF改善老年性记忆衰退的作用机制。

     

    Abstract: In the present study, the influence of direct intrahippocampal injection of NGF (nerve growth factor) on synaptosomal free Ca2+i in 1-month-old and 18-month-old mice was observed.The regulation of NGF on synaptosomal free Ca2+i in vitro was also investigated. The synaptosomal free Ca2+i in the main brain regions (cerebral cortex, hippocampus, cerebellum, diencephalon) of these mice was measured by fluorescent probe Fura-2 of Ca2+ indicator and an AR-CM-MIC cation measurement system. The results are as follows: (1) NGF showed no detectable effect on hippocampal intrasynaptosomal free Ca2+i in 1-month-old mice, but appropriate dose of NGF was shown to markedly reduce Ca2+i in 18-month-old mice. (2) Under the condition of low Ca2+i level caused by the drug, NGF was found to increase Ca2+i; conversely, was reduced Ca2+i when intracellular Ca2+ was overloaded. These results suggest that NGF has dual regulation on Ca2+ level in brain and this might be an important mechanism for its action in improving memory lesion,in aged person.

     

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