Abstract: AIM To study the chemical constituents of the root of Stellera chamaejasme L. METHODS Various column chromatographies on silica gel and RP-18 were employed for isolation and purification. Structures of compounds were elucidated by spectral analysis. RESULTS Eight lignans and three biflavonoids possessing a C-3/C-3″ linkage were isolated. They are ruixianglangdusu A (1) and B (2), 4′,4′″,5,5″,7,7″-hexahydroxy-3,3″-biflavone (3), (+)-kusunokinin (4), lirioresinol-B (5), magnolenin C (6), (-)-pinoresinol monomethyl ether (7), (-)-pinoresinol (8), (+)-matairesinol (9), isohinokinin (10) and (-)-eudesmin (11). CONCLUSION Compounds 1 and 2 are new biflavanones, 1 is enantiomeric to known chamaejasmenin C, 4, 6, 8, 9, 10 and 11 were isolated from this plant for the first time, and 7 was isolated from natural resources for the first time. In vitro bioassays showed that 3 and 8 exhibited antibacterial activity, and 1, 2, 9 and 11 exhibited immunomodulatory activity.