Abstract: A method for the quantitative determination of three main bile acids, cholic acid (CA), ursodesoxyeholic acid (UDCA) and chenodesoxycholic acid (CDCA), in bear gall, drainage from bear gall and bear gallstone is described.Experimental conditions: TLC Scanner CS-910, fluorescence scanning, λex 470 nm and λem 550 nm for CA; λex 380 nm and λem 450 nm for UDCA and CDCA.The results showed that the contents of UDCA and CDCA in bear gall drainage were higher than those in bear gall.The method is simple, rapid and sensitive. The reproducibility is good. The average recovery is 98.4%, CV is 1.4%.