Abstract: Six alkaloids were isolated from the root Zanthoxylum bungeanum Maxim. One of them, the major base is a new pyrano-2-quinolone type alkaloid zanthobungeanine and it's structure was confirmed as (Ⅲ) on the basis of spectral data. The other five were known alkaloids: 11-methoxychelerythrine (Ⅰ), des-N-methylchelerythrine (Ⅱ), arnottianamide (Ⅶ), skimmianine (Ⅵ) and L-N-acetylanonanine (Ⅳ), The last one was isolated for the first time from zanthoxylum. β-sitosterol (Ⅴ) was also isolated.Zanthobungeanine showed a wide spectrum of antifungus activity, but the action was weak.