从药代参数AUC值探讨中药复方双参通冠的配伍

Evaluation of different combinations of components of Chinese formulation Shuangshentongguan by using AUC values

摘要:
应用LC-MS/MS方法对复方双参通冠中7种成分脱氢紫堇碱、延胡索乙素、丹酚酸B、人参皂苷Rg₁、Re、Rb₁和Rd在不同配伍方式下的血药浓度进行了测定, 应用WinNonlin软件进行数据处理, 通过AUC值探讨不同配伍对这7种成分的影响。结果显示配伍后延胡索乙素的AUC值显著提高, 丹酚酸B、人参皂苷Rg₁、Re和Rd的AUC值显著降低, 人参皂苷Rb₁在两两配伍下AUC值升高, 但复方配伍给药下AUC值降低, 脱氢紫堇碱血药浓度低于检测限而无法判定配伍对其影响。

Abstract:
A sensitive liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was applied for the quantification of each component: tetrahydropalmatine (THP), dehydrocorydaline (DHC), salvianolic acid B (SAB), ginsenoside Rg₁, Re, Rb₁ and Rd in the Chinese herbal component SSTG (Shuangshentongguan) with different combinations. The pharmacokinetic data were analyzed with WinNonlin 5.2 software. The results showed that combination can increase the THP AUC value while the AUC values of SAB, ginsenoside Rg₁, Re, Rb₁ and Rd were reduced. These results showed significant differences. The AUC value of ginsenoside Rb₁ was increased when combined with Danshen or Yanhusuo, but reduced when combined with Danshen and Yanhusuo. The DHC concentration in serum was too low to be determined.