荧光光谱法研究抗癌新药吡柔比星与DNA的相互作用

STUDIES ON THE STYLE OF INTERACTION OF PIRARUBICIN WITH DNA BY FLUORESCENCE SPECTRA

摘要: 目的：研究抗癌新药吡柔比星(THP)与DNA的作用方式。方法：利用荧光光谱法和吸收光谱法，以THP为荧光探针研究测定DNA。结果：THP与DNA的结合常数为1.66×105 L.mol^-1,结合位点数为2.6 base pairs。此外,利用DNA对THP的荧光猝灭作用研究了对DNA的分析应用。DNA的检出限为0.91 μg.mL^-1,线性范围为0.91～9.0 μg.mL^-1和9.0 μg.mL^-1～26 μg.mL^-1。结论：THP的蒽醌环与DNA的碱基对发生嵌入作用,而THP的含糖侧链与DNA有小沟槽作用。

Abstract: AIM: To study the characteristics of the interaction of pirarubicin (THP) with DNA. METHODS: Study and determination of DNA with THP as a fluorescence probe was presented by absorption and fluorescence spectra. RESULTS: The binding constant of the interaction between THP and DNA is 1.66×105 L.mol^-1, and the binding site number is about 2.6 base pairs. The limit of detection is 0.91 μg.mL^-1 and the linear ranges are 0.91～9.0 μg.mL^-1 and 9.0～26 μg.mL^-1 respectively for the determination of DNA. CONCLUSION: It indicates that the anthracycline moiety of THP intercalates the stacked base pairs of DNA, which is the major controlling factor. In addition, the complex sugar structure of THP may be bound to the minor groove of DNA double helix.