流动注射分析法研究毫微粒制剂体外释药和降解

APPLICATION OF FLOW INJECTION ANALYSIS TO THE STUDIES OF IN VITRO ENZYMATIC DEGRADATION AND RELEASE OF NANOPARTICLE OR NANOCAPSULE

摘要: 应用流动注射分析技术考查了氟苷水溶液(I)、氟苷(5-氟-2'-脱氧尿苷)毫微粒(II)、氟苷酯化前体药物毫微囊(III)体外释放和III在肝、脾、肺组织提取液中的酶降解。结果表明:氟苷制成毫微粒后,体外释放变慢;而III几乎没有药物释放;III在肝、脾、肺组织提取液中皆能降解,其中主要降解场所为肝脏。开发了流动注射分析在药学领域中的新用途。

Abstract: Flow injection analysis(FIA)was appied to study the in vitro re1ease of 5-fluoro-2'-deoxyuridine(FUdR )from solution(I), FUdR-nanoparticle(II), FUdR-ester-prodrug-nanocap-sule(III)and the enzymatic degradation ofⅢ with liver ,spleen or lung extract.The results showedthat the release rate of FUdR from Il was slowed down, while the amount of drug released from IIIwas little. It was also confirmed thatⅢ could be degraded in the solution containing liver l spleen orlung extract , the major location for the degradation ofⅢ was in the liver.A new application of FIAto the pharmaceutical field was developed.