HPLC-ELSD法测定四季青中三萜及其皂苷的含量

解军波; 毕志明; 李萍

HPLC-ELSD法测定四季青中三萜及其皂苷的含量

HPLC-ELSD determination of triterpenoids and triterpenoid saponins in Ilex purpurea leaves

摘要

摘要: 目的建立同时测定四季青中3种三萜及其皂苷——长梗冬青苷、地榆皂苷I和救必应酸含量的HPLC分析方法。方法C₁₈柱；流动相：甲醇-水（59∶41）；流速1.0 mL·min^-1；检测器：Sedex 55蒸发光散射检测器。结果线性范围为长梗冬青苷2.56～25.60　μg（r=0.999.2），地榆皂苷I 1.64～16.40μg（r=0.998　2）和救必应酸3.74～37.40　μg（r=0.999.4）。平均加样回收率为长梗冬青苷96.3%, RSD　1.6%　(n=5); 地榆皂苷I 97.3%, RSD　3.8% (n=5)；救必应酸97.7%, RSD2.1%　(n=5)。3个化合物的精密度RSD　(n=6)均<4%; 重现性RSD　(n=6)均<5%。结论本方法简便、有效、可行，可用于四季青三萜及其皂苷的含量测定。

Abstract: AimTo establish an HPLC-ELSD method for the quantification of pedunculoside, ziyuglycoside I and rutundic acid, in the leaves of Ilex purpurea Hassk. MethodsBy optimizing the chromatographic conditions of HPLC and the parameters of ELSD to study the methodology. Column: Allsphere ODS-2 (250 mm×4.6 mm ID, 5 μm), mobile phase: methanol-water (59∶41), flow rate: 1.0 mL·min^-1, drift tube temperature: 59 ℃, gas flow rate: 2.38 L·min^-1. ResultsThe calibration curves were linear in the range of 2.56-25.60 μg for pedunculoside, 1.64-16.40　μg for ziyuglycoside I and 3.74-37.40　μg for rutundic acid. The average recovery of pedunculoside was 96.3%, RSD 1.59% (n=5), ziyuglycoside I 97.3%, RSD 3.82% (n=5), rutundic acid 97.7%, RSD 2.11% (n=5). All of RSDs of the precision were less than 4% (n=6), and the reproduciblities less than 5% (n=6). ConclusionThe method is simple, accurate, effective and feasible. It can be used for the determination of the contents of pedunculoside, ziyuglycoside I and rutundic acid in leaf of Ilex purpurea Hassk.

HTML全文

参考文献(0)

施引文献

资源附件(0)