Abstract: AimTo study the chemical constituents of Ardisia punctata. MethodsCompounds were separated with a combination of multi-chromatography. Their chemical structures were determined on the basis of spectral analysis and single crystal X-ray diffraction. ResultsThree compounds were isolated from chloroform extract of the roots of Ardisia punctata. Their structures were elucidated as 2-tridecyl-3-［(2-tridecyl-3-acetoxy-4-methoxy-6-hydroxy)-phenyl］-6-methoxy-1,4-benzoquinone (1), 2-tridecyl-3-［(2-tridecyl-4,6-dihydroxy)-phenyl］-6-methoxy-1,4-benzoquinone (2) and 2-tridecyl-3-［(2-pentadecyl-4,6-dihydroxyl)-phenyl］-6-methoxy-1,4-benzoquinone (3). ConclusionThe three compounds are new 1,4-benzoquinone derivatives.