杨彬, 蔡耘, 韩宗进, 武力民, 张其楷, 杨松成. 鲑鱼降钙素(sCT)类似物在水溶液中的化学稳定性J. 药学学报, 1998, 33(8): 610-615.
引用本文: 杨彬, 蔡耘, 韩宗进, 武力民, 张其楷, 杨松成. 鲑鱼降钙素(sCT)类似物在水溶液中的化学稳定性J. 药学学报, 1998, 33(8): 610-615.
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Yang Bin, Cai Yun, Han Zongjin Wu Limin, Ding Zhenkai, Zhang Qikai , Yang Songcheng, . CHEMICAL STABILITY OF SALMON CALCITONIN (sCT) ANALOGUES IN AQUEOUS SOLUTIONJ. Acta Pharmaceutica Sinica, 1998, 33(8): 610-615.
Citation: Yang Bin, Cai Yun, Han Zongjin Wu Limin, Ding Zhenkai, Zhang Qikai , Yang Songcheng, . CHEMICAL STABILITY OF SALMON CALCITONIN (sCT) ANALOGUES IN AQUEOUS SOLUTIONJ. Acta Pharmaceutica Sinica, 1998, 33(8): 610-615.
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鲑鱼降钙素(sCT)类似物在水溶液中的化学稳定性

CHEMICAL STABILITY OF SALMON CALCITONIN (sCT) ANALOGUES IN AQUEOUS SOLUTION

    摘要
  • 摘要: 用反相高效液相色谱和快原子轰击质谱方法(FAB-MS),研究了3个鲑鱼降钙素(sCT)类似物Val1,Ala7sCT,Val1,Ala7,Ala30sCT和D-Ala30sCT在不同pH和温度下水溶液中的化学稳定性,并对sCT及其类似物在pH9.0水溶液中的部分降解产物通过FAB-MS进行了确证。结果表明,sCT及其3个类似物在水溶液中的化学降解对pH依赖性较大,在碱性条件下易降解。提示不含二硫键的直链肽类似物Val1,Ala7sCT和Val1,Ala7,Ala30sCT的化学稳定性比sCT高,而D-Ala30sCT的化学稳定性则相对降低。

     

    Abstract: Calcitonin is a peptide hormone with 32 amino acids. Natural calcitonins are presumably quite unstable in solution and numerous analogues of calcitonin had been synthesized to improve the stability. In the present paper, we report the investigation of the chemical stability of salmon calcitonin (sCT) analogues Val1,Ala7sCT, Val1,Ala7,Ala30sCT and D-Ala30sCT in aqueous solutions by reversed phase highperformance liquid chromatography (RP-HPLC) and fast atom bombardment mass spectrometry (FAB-MS) methods. The degradation patterns of the sCT analogues showed a marked dependence on pH and temperature. Most of the degradation products in pH 9.0 solution of sCT and its analogues were identified by FAB-MS analysis. Results showed that the sCT analogues are more stable at pH 3.3 and pH 3.7 than at pH 6.0 and pH 9.0. Analogues without disulfide bridge Val1,Ala7sCT and Val1,Ala7,Ala30sCT showed higher chemical stability than sCT while D-Ala30sCT showed lower chemical stability than the native sCT.

     

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