Abstract: AIM To study the triterpenoid saponins in the Chinese traditional medicine Eclipta prostrata L.. METHODS Column chromatography with silica gel and HPLC were employed for the isolation and purification. The molecular structures were determined on the basis of spectral analysis (IR, MS, ¹HNMR, ¹³CNMR, HMQC and HMBC). RESULTS Two new triterpenoid saponins,named eclalbasaponins XI (4) and XII (5), were obtained and their structures were elucidated as 3-O-β-D-glucopyranosyl(1→2)-β-D-glucopyranosyl-16α-ethoxy-olean-12-ene-28-oic acid-28-O-β-D-glucopyranoside and 3-O-(2-O-sulfuryl-β-D-glucopyranosyl)(1→2)-β-D-glucopyranosyl-echinocystic acid-28-O-β-D-glucopyranoside, respectively, along with three known saponins, eclalbasaponins II (1), I (2) and III (3). CONCLUSION Compounds 4 and 5 are new compounds, 1 and 5 induced morphological deformation of Pyricularia oryzae mycelia.