Abstract: Four crystalline and one liquid compounds were isolated from the solid cultured extract of Hericium erinaceus, Two of them are new, named herierin Ⅲ and Ⅳ. Their chemical structures were determined as 6-methy 1-2,5-dihydroxymethyl-γ-pyranone (Ⅲ) and 2-hydroxymethy 1-5-a-hydroxy-ethyl-γ-pyranone (Ⅳ). The others are 4-chloro-3, 5-dimethoxybenzoic-O-arabitol ester, 4-chloro-3, 5-dimethoxybenz0ic methyl ester and 4-chloro-3,5-dimethoxybenzoic acid. In addition, a mixture of palmic and stearic acid, a mixtrue of behenic acid and tetracosanic acid and a mixture of 5α-ergostan-3-one, 5α-stigmasten-22-en-3-one and 5α-stigmastan-3-one were also isolated.