HPLC法测定中药莪术中3种姜黄素的含量

HPLC DETERMINATION OF THREE CURCUMINOID CONSTITUENTS IN RHIZOMA CURCUMAE

摘要: 目的：用高效液相色谱法测定中药莪术中3种姜黄素成分即姜黄素(curcumin, I)、脱甲氧基姜黄素(demethoxycurcumin, II)和双脱甲氧基姜黄素(bisdemethoxycurcumin, III)。方法：采用Inertsil ODS-3色谱柱，流动相为甲醇—异丙醇—水—冰乙酸(20∶27∶48∶5)，流速为0.5 ml.min^-1，检测波长为420 nm。结果：3种姜黄素在0.1～1.9 μg呈良好线性关系，方法回收率为98.2%～101.1%，曾用本法测定了8种莪术样品。结论：本法准确，快速，重现性好，可用于考察研究中药莪术的不同品种以及同属其它中药的质量。

Abstract: AIM: To determine curcuminoid constituents including curcumin, demethoxycurcumin and bisdemethoxycurcumin of rhizoma Curcumae. METHODS: A reversed-phase HPLC system consisting of an ODS column and a mixture of methanol — isopropanol — water — acetic acid (20∶27∶48∶5) as the mobile phase was used. The flow rate was 0.5 ml.min^-1 and detection was effected at 420 nm. RESULTS: The assay displayed good linearity over the concentration range of 0.1～1.9 μg and recoveries were 98.2%～101.1%. CONCLUSION: The method is accurate, rapid and reproducible and can be used for the quality study of rhizoma Curcumae.