Abstract: AIM: To set up euglycemic clamp technology. METHODS: The availability of One touch II was investigated by regression analysis and coefficient of variation. The insulin infusion was prepared by diluting insulin with 6% hydroxyethylamylin(0.9% NS) solution. Shifts of blood glucose were determined either in the change of insulin infusion rate or in the change of glucose infusion rate. RESULTS: One touch II was available for the euglycemic clamp because of its good correlation and precision. The insulin infusion diluted by hydroxyethylamylin was available for the clamp. Steady state blood glucose was controlled in basal±0.2 mmol.L^-1. Blood glucose value was more sensitive to glucose infusion rate than insulin infusion rate. CONCLUSION: For the first time in China, euglycemic clamp technique in rats had been set up with high sensibility and good stability.