Abstract: An alkaloid and a quassinoid were isolated from the fruit of Brucea javanica (L.) Merr, and were identified as 4-ethoxycarbonyl-2-quinolone (1) and bruceine Ⅰ (2). Compound 1 was found from natural resource for the first time. The structure of the new compound 2 was elucidated on the basis of spectral data and chemical evidence. Three well known compounds vanillic acid (5), quercetin-3-O-β-D -galactoside(4), and luteolin-7-O-β-D-glucoside (5) were also isolated. All of them were first reported to occur in the Brucea genus.