莽草酸对血小板聚集和凝血的抑制作用

INHIBITORY EFFECTS OF SHIKIMIC ACID ON PLATELET AGGRAGATION AND BLOOD COAGULATION

摘要: 目的：研究莽草酸(SA)对血小板聚集性的影响及其机制，并研究其对凝血时间的影响。方法：采用比浊法测定血小板聚集功能，放射免疫法测定血小板TXB2和血浆6-keto-PGF_1α水平，玻管法测定凝血时间。结果：SA体外呈浓度依赖性抑制ADP、胶原诱导的兔血小板聚集，IC₅₀分别为9.25, 3.56 mmol.L^-1；在体内(ip)抑制脑血栓模型鼠由ADP诱导的血小板聚集; SA促进大鼠血浆6-keto-PGF_1α的生成并延长小鼠凝血时间。结论：SA对血小板聚集及凝血系统有显著的抑制作用。

Abstract: AIM：To study the inhibitory effects of shikimic acid (SA) on platelet aggregation and its mechanism, and the effect on coagulation time. METHODS: Methods of turbidimetry and radioimmunoassay were used to measure platelet aggregation and the levels of thromboxane B₂(TXB₂) and 6-keto-PGF1α. RESULTS: SA inhibited rabbit platelet aggregation induced by ADP and collagen in vitro with IC₅₀ of 9.25 and 3.56 mmol.L^-1, respectively. SA 25 and 50 mg.kg^-1 ip for 3 d inhibited platelet aggregation induced by ADP in rats subjected to middle cerebral artery thrombosis(MCAT). SA was shown to increase rat plasma 6-keto-PGF_1α and no significant effect on TXB2 generation in PRP induced by ADP. SA 25, 50 and 100 mg.kg^-1 im or iv elevated the blood coagulation time of mice.　CONCLUSION: SA inhibited platelet aggregation and blood coagulation.