齐墩果酸通过VEGF途径抑制内皮细胞的迁移和管腔形成

魏鉴腾; 刘 明; 刘海洲; 赵 进; 肖 琳; 韩丽君; 林秀坤

齐墩果酸通过VEGF途径抑制内皮细胞的迁移和管腔形成

Oleanolic acid inhibits proliferation of HUVECs, and inhibits migration and tube formation via VEGF pathway

摘要

摘要:
为研究齐墩果酸 (OA) 对内皮细胞的迁移和管腔形成的影响及其作用机制, 采用MTT法、平板划痕、Transwell小室和体外管腔形成实验检测OA对HUVECs增殖、迁移和管腔形成的影响。MTT分析结果显示, OA能够抑制HUVECs增殖, 并且呈现时间和浓度依赖性关系。平板划痕和Transwell小室结果显示, 40 μg·mL⁻¹ OA细胞的迁移率分别为71%和87%。管腔形成实验证明, OA的质量浓度达到40和60 μg·mL⁻¹时, 管腔形成系数为33% 和20%。Western blotting检测发现, OA能够抑制HUVECs VEGF的表达和分泌。另外, VEGF可以部分逆转OA对HUVECs的迁移与管腔形成的抑制作用。综上所述, OA可以通过VEGF介导的信号途径抑制细胞的迁移和管腔形成。

Abstract:
To investigate the effects of oleanolic acid (OA) on the proliferation, migration and the formation of tube-like structure in human vascular endothelial cells (HUVECs). MTT assay, flat plate scarification, Transwell plates and matrigel-induced tube formation assay were performed to detect the effects of OA on proliferation, migration and tube formation. MTT assay showed that the inhibition rates of HUVECs treated with 60 and 100 μg·mL⁻¹ of OA for 24 h were 19% and 83% respectively. Treatment of HUVECs significantly inhibited the cell migration in a dose-dependent manner. The vascular indexes of HUVECs treated with 40 and 60 μg·mL⁻¹ OA were 33% and 20% respectively. Western blotting analysis showed that treatment of the cells with OA significantly attenuated the expression and secretion of VEGF. Additionally, VEGF could in part reverse the effects of OA on migration and tube formation of HUVECs. In conclusion, OA inhibits the proliferation, and VEGF plays an important role in OA induced decreased migration and tube formation of HUVECs.

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