Abstract: Seven known alkaloids, aconitine, mesaconitine, hypaconitine, delphline, delcosine, methyllycaconitine, and delsemine, were employed for the study of thin-layer chromato- graphic separation and identification of aconite alkaloids. It was found that basic alumina of activity Ⅳ with partide size smaller than 150 mesh was a suitable absorbent, and a mixture of petroleum ether and ether (1: 10) was a suitable developing agent. The re- sults in the present work indicated that thin-layer chromatography was a satisfactory method for rapid identification of alkaloids of aconite including Chinese aconites.