Reversible protein phosphorylation regulates multiple biochemical events.  Mycobacterium tuberculosis phosphatases play important roles in regulating the pathogen physiology and interference of host signaling.  They are also involved in the evasion of host immune response and blockage of the phagosome- lysosome fusion.  Selective inhibition of phosphatase represents an ideal new avenue of anti-tuberculosis drug design.  In this paper, we update the progresses about the regulation network of Mycobacterium tuberculosis phosphatases including MptpA, MptpB, MstP, SapM and their inhibitors.  These serve as the basis for further anti- tuberculosis drug target.