Abstract: A new and a known diterpenoid alkaloid have been isolated from the root of Delphinium kamaonense var. glabrescens. The chemical structure of the new alkaloid, named glabredelphinine, was established as 2 (3)-dehydro-6-demethyl-18-demethoxy-delcosine by IR, MS, ¹HNMR and ¹³CNMR spectral data. The known one was identified as tatsiensine. They were isolated from this plant for the first time.