大鼠重复注射米托蒽醌热敏脂质体产生ABC现象

Repeated injection of mitoxantrone containing thermosensitive liposomes in rat induced ABC phenomenon

摘要: 考察大鼠重复注射米托蒽醌热敏脂质体是否产生加快血液清除现象（accelerated blood clearance phenomenon，ABC phenomenon）。液质联用测定大鼠血浆中米托蒽醌浓度，并对血浆样品中抗聚乙二醇（polyethylene glycol，PEG）IgM抗体进行酶联免疫吸附剂测定（enzyme linked immunosorbent assay，ELISA）的检测。结果显示，第2次给药后药物清除速率变快，给药前血浆中抗PEG IgM抗体在第2次给药后被迅速中和。证明大鼠重复注射米托蒽醌热敏脂质体会产生ABC现象。

Abstract: To investigate whether accelerated blood clearance (ABC) phenomenon could be induced after repeated injection of mitoxantrone thermosensitive liposomes, LC-MS/MS and enzyme linked immunosorbent assay (ELISA) were used to measure the concentration of mitoxantrone and the anti-polyethylene glycol (PEG) IgM levels in rat plasma, separately. The drug was rapidly cleared away after the second administration. The anti-PEG IgM was detected after the first dose which was neutralized quickly after the second dose. It is proved that repeated administration of mitoxantrone thermosensitive liposomes in rat caused the ABC phenomenon.