14C-棉酚:棉花幼苗生物合成的最佳条件(英文)
14C-GOSSYPOL:OPTIMUM CONDITIONS FOR SYNTHESIS BY COTTON SEEDLINGS
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摘要: 将1-14C-乙酸钠加入棉花(Gossypium hirsutum c.v.ST-506)幼苗的培养液中,经四天的培育,得到最高放射活性的棉酚。实验中亦显示体外加乙酸钠具有激发棉酚生物合成的特性。每两百株棉花幼苗加入6 mg乙酸钠可得最高量的棉酚。Abstract: The biosynthesis of 14 C-gossypol from sodium 1-14C-acetate has been studied in 6 day old seedlings of the cotton plant, Gossypium hirsutum L.c.v. ST-506. The highest specific activity gossypol (0.8μCi/m mole) was obtained when seedlings were incubated for 96 hr in the presence of 6 mg/200 seedlings of sodium 1-14C-acetate (0.4 mCi/m mole) in a defined nutrient medium.
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