两种测定小鼠体内力达霉素药代动力学方法的比较

Comparison of the pharmacokinetics of lidamycin in mice determined by two methods

摘要: 目的对测定小鼠血清浓度的总放射性方法和经过高效液相色谱分离后再测定放射性的两种方法进行比较。方法首先对力达霉素进行同位素标记并且分离纯化。给小鼠尾iv ¹²⁵I-力达霉素(100 μg·kg^-1)，采集血样，样品经两种方法测定。对得到的药代动力学参数进行统计学处理。结果两种方法测定得到的药代动力学参数(V_d, t_1/2α, t_1/2β, k₂₁, k₁₀, k₁₂, AUC和CL) 存在显著性的差异 (P<0.05)。结论采用色谱分离后再测定¹²⁵I-力达霉素原形的放射性较为合理和准确。

Abstract: AimTo compare two methods, the total radioactivity assay (RA method) and the radioactivity assay after separation with high performance liquid chromatography (HPLC-RA method). Methods¹²⁵I-Lidamycin was prepared by Iodogen method and separated by size exclusive high performance liquid chromatography. The pharmacokinetic parameters of lidamycin were assayed by two methods after intravenous injection to mice at the dose of 100 μg·kg^-1, and compared by statistical analysis. ResultsThe pharmacokinetic parameters (_V_d, t_1/2α, t_1/2β, k₂₁, k₁₀, k₁₂, AUC and CL) showed significant difference between the two methods (_P<0.05). ConclusionThe HPLC-RA method was better than the RA method to determine unchanged ¹²⁵I-lidamycin.