薄层层离法在研究天然化合物中的应用Ⅶ.挥发油中主要成分的鉴定
THIN LAYER CHROMATOGRAPHY APPLIED TO NATURAL PRODUCTS Ⅶ. IDENTIFICATION OF THE PRINCIPAL CONSTITUENTS IN ESSENTIAL OILS
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摘要: 本文研究了加硫酸钙为粘合剂的氧化铝薄层板的制备方法和影响挥发油中成分分离的各种因素.试验指出,用通过120号筛孔的中性氧化铭(5份),硫酸钙(1份),水(7份)调制成糊,涂于玻璃板上,再于120℃烘烤2小时即制得良好的薄层板,适于挥发油中主要成分的分离和鉴定.作者还观察了10种显色剂对各类成分的显色情况,并且应用本文方法对12种挥发油中主要成分进行鉴定,均获得良好的结果.此外,对30种中药的挥发油制出了薄层层离谱.Abstract: 1.The present paper presents a method for preparing chromatostrip by coating one side of a glass strip with a paste of neutral alumina, gypsum, and water in the propor- tion of 5:1:7. The thickness of the paste is 1 mm, and the paste is afterward dried for 2 hours at 120℃. This gives a uniform thin layer which is suitable for the separa- tion and identification of the principal chemical constituents in essential oils. 2. By using the chromatostrips, the author succeeded in identifying the principal chemical constituents in 12 essential oils, and presented a series of thin layer chromato- graphs for 30 aromatic plants used in Chinese medicine.
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