羟基红花黄色素A对血小板活化因子的拮抗作用

ANTAGONISTIC EFFECT OF HYDROXYSAFFLOR YELLOW A ON THE PLATELET ACTIVATING FACTOR RECEPTOR

摘要: 目的观察羟基红花黄色素A(HSYA)对血小板活化因子(PAF)的拮抗作用.方法以放射受体结合试验观察HSYA抑制3HPAF与家兔洗涤血小板(WRP)特异性结合的作用,以比浊法观察HSYA抑制PAF介导的WRP及兔多型核白细胞(PMNs)聚集的作用.结果 HSYA可浓度依赖地抑制1,2及4 nmol·^L-1 ³HPAF与WRP受体的结合;HSYA抑制PAF介导的WRP及兔PMNs聚集,均具明显的量效关系,其IC₅₀分别为0.99及0.70 mmol·L^-1.结论 HSYA有PAF受体拮抗作用.

Abstract: AIMTo observe the antagonistic effect of hydroxysafflor yellow A (HSYA) on the platelet activating factor (PAF). METHODSWashed rabbit platelet (WRP) aggregation and rabbit polymorphonuclear leukocytes (PMNs) aggregation induced by PAF were observed by turbidimetric assay in vitro. The PAF receptor antagonistic effect of HSYA was investigated by radio ligand binding assay (RLBA). RESULTSIn RLBA the specific binding inhibition effect of HSYA was found to be concentration-dependent in three different ［³H］PAF concentrations. In the experiments, WRP aggregation and rabbit PMNs aggregation induced by PAF (9.55×10^-10, 9.55×10^-6 mol·L^-1) were both inhibited by HSYA in a concentration-dependent manner in vitro. The IC₅₀ of HSYA to inhibit WRP and rabbit PMNs aggregation was 0.99 and 0.70 mmol·L^-1, respectively. CONCLUSIONThe PAF receptor binding can be antagonized by HSYA.