Abstract: This paper deals with the optimum culture medium inducing callus from excised Collars, i. e. buds on the root tips and excised petiole segmenes of Angelica sinesis and with the effect of hormones and difference in temperature upon the organic differentiation from callus. Cytological observation demonstrated that the plant regeneration from callus were completed in two ways, the formations of embryoid and adventitious bud. The methods of thin-Layer chromatography and paper chromatography have been used to identify the ability of the cultures to synthesize essential oil and carbohydrate. The result shows that the cultures may produce the same essential oil and carbohydrate as the root of cultivated Angelica sinesis does.