Abstract: Application of thin layer chromatographic method to separation and subsequent visible densitometric or UV spectrophotometric determination of vincamine in Vinca minor is described.By densitometric method the linear range was 0.5～3.0μg, per spot and CV% of pure vincamine was 2.02 (n=9).Beer's law was obeyed in the range of 50～250μg in 5 ml of 1 N hydrochloric acid solution and recovery of pure vincamine was 98.2±2.1% by the UV spectrophotometric method.The results of quantitative determination of 6 samples by both methods agreed very well, but. the densitometric method was far more rapid and sensitive than the UV method.