王亚明, 周开亚, 吴平, 徐珞珊. 中药材龟板和鳖甲中DNA的提取与扩增J. 药学学报, 1996, 31(6): 472-476.
引用本文: 王亚明, 周开亚, 吴平, 徐珞珊. 中药材龟板和鳖甲中DNA的提取与扩增J. 药学学报, 1996, 31(6): 472-476.
YM Wang, KY Zhou, P Wu , LS Xu, . ISOLATION AND AMPLEFICATION OF DNA FROM TORTOISE AND TURTLE SHELLSJ. Acta Pharmaceutica Sinica, 1996, 31(6): 472-476.
Citation: YM Wang, KY Zhou, P Wu , LS Xu, . ISOLATION AND AMPLEFICATION OF DNA FROM TORTOISE AND TURTLE SHELLSJ. Acta Pharmaceutica Sinica, 1996, 31(6): 472-476.

中药材龟板和鳖甲中DNA的提取与扩增

ISOLATION AND AMPLEFICATION OF DNA FROM TORTOISE AND TURTLE SHELLS

  • Abstract: DNA was extracted from tortoise and turtle shells, two Chiense crude drugs whichhave been preserved for at least nine years.The cytochrome b sequence was amplified from theextracts using the polymerase chain reaction,Treatments with detergent and ultraviolet light wereemployed to destroy possible extraneous contamination,A control extract was set in parallel with theextracts of the specimens in order to detect contamination in solutions and reagents.The results showthat significant amounts of genetic information ean survive in the shells and may have importantimpllcations for their identification.

     

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