Abstract: AimTo study the chemical constituents of Dioscorea parviflora. MethodsThe chemical constituents were isolated by silica gel and RP-18 column chromatography, and their chemical structures were elucidated by IR, NMR and MS. ResultsEleven steroides have been isolated from EtOH extract of Dioscorea parviflora and identified as isonarthogenin 3-O-α-L-rhamnopyranosyl-(1→2)- β-D-glucopyranoside (I), diosgenin-diglucoside (II), prosapogenin A of dioscin (III), dioscin (IV), deltonin (V), deltoside (VI), methyl deltoside (VII), diosgenin 3-O-β-D-glucopyranosyl (1→3)- β-D-glucopyranosyl-(1→4)-［ α-L-rhamnopyranosyl-(1→2)］- β-D-glucopyranoside (VIII), parvifloside (IX), methyl parvifloside (X), diosgenin (XI), and a mixture of β-sitosterol and stigmasterol. ConclusionCompound X is a new compound. Compounds VII and X were methyl ethers of VI and IX, respectively, and may be produced during isolation. Compound I is isolated from Dioscorea L., and II, IV from Dioscorea parviflora for the first time.