Chemical constituents of the roots and stem of Salacia hainanensis Chun et How were isolated and purified with column chromatography on silica gel, Sephadex LH-20 and preparative HPLC.  Their structures were elucidated based on physicochemical and spectral spectroscopic analysis.  Depending on the activities of anti-α-glucosidase and inhibiting AGEs (advanced glycation end products, AGEs) formation in vitro, nine   compounds were identified as 26, 27-dihydroxy-7, 24-tirucalladien-3-one (1), abruslactone A (2), lupeol (3),  21α, 30-dihydroxy-D: A-friedooleanan-3-one (4), 15α-hydroxyfriedelan-3-one (5), friedelin (6), mangiferin (7), epicatechin (8) and β-sitosterol (9), separately.  Among them, compound 1 is a new compound, and compound   2 was isolated from the Salacia genus for the first time, while, compounds 3, 4, 5, 8 were obtained from this plant for the first time.