李斌, 谢笔钧. 魔芋葡甘聚糖分子链形态及链参数研究魔芋葡甘聚糖分子链形态及链参数研究J. 药学学报, 2003, 38(11): 834-842.
引用本文: 李斌, 谢笔钧. 魔芋葡甘聚糖分子链形态及链参数研究魔芋葡甘聚糖分子链形态及链参数研究J. 药学学报, 2003, 38(11): 834-842.
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LI Bin, XIE Bi-jun. Study on molecular chain morphology and chain parameters of konjac glucomannanJ. Acta Pharmaceutica Sinica, 2003, 38(11): 834-842.
Citation: LI Bin, XIE Bi-jun. Study on molecular chain morphology and chain parameters of konjac glucomannanJ. Acta Pharmaceutica Sinica, 2003, 38(11): 834-842.
shu

魔芋葡甘聚糖分子链形态及链参数研究魔芋葡甘聚糖分子链形态及链参数研究

Study on molecular chain morphology and chain parameters of konjac glucomannan

    摘要
  • 摘要: 目的研究魔芋葡甘聚糖分子链形态，测定分子链参数。方法采用光散射、凝胶渗透色谱及粘度法测定溶液行为；采用原子力显微镜及透射电镜直接观测分子形貌。结果魔芋葡甘聚糖重均分子量Mw、均方根旋转半径〈S2〉1/2以及第二维利系数A2分别为1.04×106, (105.0±0.9) nm,和 (-1.59±0.28)×10-3 mol·mL·g-2，多分散系数Mw/Mn为1.02，Mark-Houwink方程为［η］=5.96×10-2M0.73w；分子链参数ML=982.82 nm-1,q=27.93 nm, d=0.74 nm,h=0.26 nm,L=1 054.11 nm。结论直接观察到的分子形貌和根据溶液行为的推测均说明，魔芋葡甘聚糖为伸展的有一定刚性的半屈曲性直链分子，不存在支链。

     

    Abstract: AimTo study the molecular chain morphology and chain parameters of konjac glucomannan(KGM). MethodsSolution behavior was studied by using light scattering(LS), gel permeation chromatograph (GPC) and method of viscosity. The molecular morphology was observed directly by atom force microscope (AFM) and transmission electron microscope (TEM). ResultsThe average molecular weight (Mw), root-mean-square ratio of gyration(〈S2〉1/2), second Viral coefficients (A2) and multi-disperse coefficients (Mw/Mn) are 1.04×106, 105.0±0.9 nm, (-1.59±0.28)×10-3 mol·mL·g-2 and (1.020±0.003) respectively; Mark-Houwink equation was established as ［η］=5.96×10-2 M0.73w, the molecular chain parameters were as follows: ML=982.82 nm-1, q=27.93 nm, d=0.74 nm, h=0.26 nm, L=1　054.11 nm. ConclusionBoth the result showed by direct observation and the deduction drawn by solution behavior confirmed that the KGM molecular is stentering semi-flexible linear chain without branch.

     

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