Abstract: Current quality control patterns are limited to industrial application, for most of the natural chemical reference substances are expensive and unavailable. Herein, a method, quantitative analysis of multi-components with single marker (QAMS), was established and validated to simultaneously determine nine ginsenosides (ginsenoside Rg₁, Re, Rf, Rh₁, Rb₁, Rc, Rb₂, Rb₃, Rd) in P.ginseng and four ginsenosides (ginsenoside Rg₁, Rh₁, Rb₁, Rd) in P.notoginseng. Using ginsenoside Rb₁ as the contrast, the relative correction factors (RCF) of the other eight ginsenosides were determined by HPLC-DAD. Within the linear ranges, the values of RCF of ginsenoside Rb₁ to ginsenoside Rg₁, Re, Rf, Rh₁, Rc, Rb₂, Rb₃ and Rd were 1.400, 1.215, 1.517, 1.801, 0.944, 1.012, 1.143, and 1.135, respectively. The RCF had a good reproducibility in various instruments, chromatographic columns (RSD=0.30%-3.9%). According to their RCF, we simultaneously determined nine ginsenosides in P.ginseng only using one marker. In addition, the RCF of ginsenosides were used to simultaneously quantitative analysis of four ginsenosides in P.notoginseng. The results of QAMS method were validated by comparing with that of external standard method, and no obvious significant difference was found.