藁本中5种成分的高效液相色谱法测定

HPLC DETERMINATION OF FIVE CONSTITUENTS IN PLANTS OF GENUS LIGUSTICUM

摘要: 报道了藁本中5种成分:阿魏酸(ferulicacid)、莨菪亭(scopoletin)、正丁苯酞(3-butyl-phathalide)、藁本内酯(ligustilide)、藁本内酯二聚体(diligustilide)的反相高效液相色谱法测定。采用梯度洗脱法,用Hypersil-C₁₈键合相柱,乙腈(含1.25%CHCl₃)—水(以CHCl3饱和)为流动相,苄噻嗪为内标,284nm为检测波长,二极管阵列检测器检测峰纯度,方法快速、简便。

Abstract: A RP HPLC method was established to separate and determine five constituents in plants of genus Ligusticum by using an ODS column (Hypersil-C₁₈ , 5 μm) and acetonitrile (containing 1 25% CHCl₃) water (saturated with CHCl₃) as mobile phase for gradient elution. The five constituents were ferulic acid, scopoletin, 3-butyl phthalide, ligustilide, and diligustide. They were detected at 284 nm. Peak purity was monitored by photodiode array detector. Benzthiazide was used as the internal standard. The method is simple, fast, sensitive and reproducible.