Abstract: An acid-dye colorimetric method was reported for the determination oftotal alkaloids in 53 samples of Baibu drugs from their growing destricts in 14 provincesand municipalities and its average recovery and its linear range were 96. 1% ( CV <4%)and 20～150μg respectively. The relationship between the total alkaloid content, geogra-phical origins and morphology were discussed. The results showed that: 1. the content of totalalkaloids of stemona was 0.26～3. 1 %; 2. that of Stemona sessilifolia was 0.26～2.17%with the highest content of the sample from Nanyang country in Henan Province; 3.that of S. japonica was 0.83～1.43%; 4. that of S. tuberosa was 0.53～3.1% with thehighest content of sample from Hengyang in Hunan Province; 5.that of S.parviflora was 0.22～0.74% with the highest content of sample from Qongzhong in Hainan Province;and 6.thatof more yellow, solider and stronger samples was higher than that of any other samples.Howerver, that of all bigger samples in shape was not higher than that of smaller ones.