共轭方向法在HPLC流动相条件优化中的应用——麻黄碱、伪麻黄碱的分离

USE OF THE POWELL METHOD TO OPTIMIZE THE COMPOSITION OF HPLC MOBILE PHASE THE SEPARATION OF EPHEDRINE AND PSEUDOEPHEDRINE

摘要: 麻黄碱与伪麻黄碱是中药麻黄的主要有效成分,也是一对化学物理性质很相似的差向异构体。二者的结构只是构象不同,用色谱方法进行分离有一定的难度。目前已有在苯基和腈基键合固定相上进行HPLC分离的报道^(1,2)。本文成功地引入共轭方向法在μBondapak C₁₈色谱柱上对流动相组成进行了条件优化,优化后的色谱条件下麻黄碱与伪麻黄碱的分离情况令人满意。

Abstract: The isomers ephedrine and pseudoephdrine are difficult to be separated by HPLC. In this paper, the Powell method was successfully introduced to optimize the composition of mobile phase in HPLC. On a μ Bondapak C₁₈ column (8～10μm, 3.9mm×30cm), KH₂PO₄ solution methanol mobile phase system was optimized forseparating ephedrine and pseudoephedrine by the Powell method. The optimized composition of the mobile phase was found as: 0.01mol/L KH₂ PO₄—MeOH=94: 6 (V/V). The Powell method was carried out in a process illustrated by tables and figures and 0.95 peak separation function was achieved.