雷公藤中二萜内酯类成分的研究 (Ⅱ)

Diterpene constituents of Tripterygium willfordii (Ⅱ)

摘要:
为对雷公藤 (Tripterygium willfordii Hook.f) 进行植物化学和药理的研究, 应用现代色谱技术对雷公藤植物的化学成分进行了提取分离, 应用UV、IR、MS、HR-MS、¹H NMR、¹³C NMR、¹H-¹H COSY、¹H-¹³C COSY和NOESY技术对分离得到的4个化合物进行结构确证。4个化合物分别归属为: 化合物1为雷公藤内酯酮 (triptonide)、化合物2为新雷公藤内酯四醇 (neo-triptetraolide)、化合物3命名为2α-羟基雷公藤内酯酮 (2α- hydroxytriptonide) 和化合物4命名为15-羟基雷公藤内酯酮 (15-hydroxytriptonide)。其中化合物3、4为新的二萜内酯并对K562细胞 (慢性粒细胞白血病细胞) 及HL60细胞 (急性髓性白血病细胞) 具有显著抑制作用。

Abstract:
In order to study the constituents and pharmacology of Tripterygium plants (Tripterygium willfordii Hook.f), a variety of chromatography methods were used. Four compounds were isolated from Tripterygium plant and their structures were elucidated by UV, IR, MS, HR-MS, ¹H NMR, ¹³C NMR and 2D-NMR techniques. The isolated compounds were named as triptonide (1), neo-triptetraolide (2), 2α-hydroxytriptonide (3), and 15-hydroxytriptonide (4), separately. Compounds 3, 4 belong to new diterpenoids, which can inhibit the growth of K562 cells (leukemia cells) and HL60 cells (acute myeloid leukemia cells).