Abstract: In continuing our studies on the flavonoids from Scutellaria amoena G.H.Wright, a new flavanone (Ⅰ)and six known compounds (Ⅱ～Ⅶ) were isolated from the roots of this plant. On the basis of spectroscopic analysis (UV,¹H NMR, ¹³C NMR, MS and CD) and chemical evidence, the structure of the new compound was elucidated as (2S)2',5,6'-trihydroxy-7-methoxyflavanone (Ⅰ) and named scuteamoenin, the other six known compounds were identified as (2R,3R)-3,5,7-trihydroxyflavanone (Ⅱ), 2',3,5,6',7-pentahydroxyflavone (Ⅲ), 2',5,7-trihydroxy₆methoxyflavone (Ⅳ), skullcaflavone Ⅱ (Ⅴ) , chrysin (Ⅵ) and β-sitosterol (Ⅶ) respectively. Compounds Ⅱ-Ⅶ were obtained from this plant for the first time.