用油/水界面伏安法测定乙酰螺旋霉素

DETERMlNATION OF ACETYLSPIRAMYCIN BY VOLTAMMETRY AT OIL / WATER INTERFACE

摘要: 在0.lmol/LCdCl_{22+离子在界面传递的伏安波。其特性与常规半微分极谱波类似。△ψ随乙酰螺旋霉素的浓度增加向阴极方向移动。ep乙酰螺旋霉素的浓度呈线性相关,线性范围为29～437U/m1,检测限为7U/ml。重现性的相对标准偏差为3%左右,回收率为97～102%。与生物法相比,测定样品结果的相对误差在±1%以内。}

Abstract: The voltammetric behavior of acetylspiramycin at water-nitrobenzerne interface have been studied by semi- differential cyclic voltammetry.The experimental results showed that acetvl-spiramycin facilitated the transfer of Cd²⁺ ions in the system of 0.1 mol/L CdCl₂(water)一0.005mol/L tetraphenylarsonium一tetraphenylborate(TPAsTPB)( nitrobenzene ,NB )and the characteris-tics of the voltammogram are the same as those of normal neopolarography.The peak current isdirectly proportional to the concentration of acetylspiramycin(NB)in the range of 29～437μ/ml andthe relative standard deviation is about 2%. No significant difference was found as compared with thebiological method.