Abstract: AIM To isolate the components from the volatile oil of Foemiculum vulgare Mill. METHODS According to the function of molecular recognition of supramolecular chemistry, chela shape molecule, trans-1,2-biphenyl-1,2-acenaphthendiol was used as host molecule and the volatile oil of Foemiculum vulgare Mill as guest molecule. Trans-1,2-biphenyl-1,2-acenaphthendiol can recognize the components that endowed with interactional complementarity and form inclusion compound as crystals. RESULTS The anethole in the volatile oil was selectively included as trans-1,2-biphenyl-1,2-acenaphthendiol which was obtained in pure state from the inclusion compound by Kugelrohr vacuum technology. The formation of inclusion compound was confirmed by means of IR and powder XRD. The structure of the selectively isolated component was elucidated as trans-anethole by means of IR, ¹HNMR and MS. CONCLUSION The experimental results showed that the method is simple, rapid and selective for isolation anethole from volatile oil of Foemiculum vulgare Mill.