Abstract: A sensitive and simplified high performance liquid chromatographic procedure has been developed for quantification of lomefloxacin in human plasma. The recovery of lomefloxacin was 95 to 102 %. The relative standard deviation was 2. 1 to 7. 8 %. The calibration curve was linear in the range from 0. 125 μg/ml to 5. 012 μg/ml with r=0. 9998. The detection limit of the method is 25 ng/ml. The plasma drug concentration--time course after medication conformed to a 1-compartment open model with a first order absorption. Mean t_1/2 value was 5.5 h.