红花RP-HPLC指纹图谱的建立及其质量研究红花RP-HPLC指纹图谱的建立及其质量研究

Establishment of chromatographic fingerprint and quality assessment of Carthamus tinctorius L. by high performance liquid chromatography

摘要: 目的建立红花RP-HPLC指纹图谱分析法,研究不同产地红花药材的质量。方法采用梯度洗脱的方法进行色谱分离,使用“计算机辅助相似度评价软件”进行数据处理,对不同产地红花药材指纹图谱的相似度进行比较分析。结果不同产地红花药材指纹图谱相似度较好,但仍有少数产地的药材指纹图谱中有明显差别。结论采用RP-HPLC方法控制药材的指纹图谱,方法重现性好,用于红花的质量评价切实可行。不同产地红花药材化学组成相似,其相对比例较稳定。

Abstract: AimTo establish chromatographic fingerprint of Carthamus tinctorius L. by RP-HPLC in order to control the quality of Carthamus tinctorius L. MethodsThe gradient elution mode was applied in chromatographic separation, and data were analysed by “Computer Aided Similarity Evaluation” software to compare the quality of Carthamus tinctorius L. samples from different habitats. ResultsSamples from different habitats were of high similarity, though a few samples showed evident difference in fingerprint graphics. ConclusionThe RP-HPLC fingerprint method is repeatable, feasible in analysis of Carthamus tinctorius L. and can be used in quality assessment of Carthamus tinctorius L.. Chemical components in Carthamus tinctorius L. samples from various habitats are similar, and their ratios between each other are stable.