Abstract: AimTo determine the entrapment efficiency of breviscapine nanoliposomes. Methods Retrodialysis method was used to determine the entrapment efficiency of breviscapine nanoliposomes. The drug recovery and the sample recovery were considered to verify the feasibility of the method. Results The drug recovery was (99.6±0.6)%. The sample recovery was (99.9±0.8)%. The entrapment efficiency of breviscapine nanoliposomes was (75.3±2.3)%. ConclusionThe method used in this study is simple, applicable and accurate for determination of the entrapment efficiency of breviscapine nanoliposomes.