Abstract: AIM　To test whether schisanhenol could inhibit oxygen free radical generation and lysosomal enzyme release from rat peritoneal neutrophils in response to different stimulants. METHODS　The production of oxygen free radical and the release of lysosomal enzymes and cytosolic ［Ca²⁺］i were studied with spectrometric and spectrophotofluorometric methods. Intracellular cAMP was measured by using isotope. RESULTS　Schisanhenol(SAL) decreased superoxide anion and hydrogen peroxide production of rat peritoneal neutrophils stimulated by formyl-Met-Leu-Phe(FMLP) and phorbol-1,2-myristate-1,3-acetate(PMA). SAL was found to inhibit lipid peroxidation of rat peritoneal neutrophils induced by Fe²⁺/L-cysteine and the release of lysozyme and β-glucuronidase after stimulation with FMLP. SAL decreased the cytosolic ［Ca²⁺］i and increased the intracellular cAMP level of the neutrophils.CONCLUSION　SAL was shown to modify the functions of rat neutrophils through affecting the cytosolic free calcium and cAMP level besides its antioxidant activity.