RP-HPLC法测定重楼中甾体皂甙的含量

DETERMINATION OF STERIUDAL SAPONINS IN RHIZOMA PARIDIS BY RP-HPLC

摘要: 为寻找和合理利用植物资源及控制生药质量,用RP-HPLC法分离并测定了重楼中9种甾体皂甙,其中4种为偏诺皂甙元的糖甙,5种为薯芋皂甙元的糖甙。分析柱为C₈(4.6mm×250mm),乙腈—水(42∶58)为流动相,检测波长203nm,9种甾体皂甙峰与其相邻峰能达基线分离,且线性良好,γ=0.9996～0.9999,回收率97.1%～100.2%。本法简便、快速、灵敏。用此法对不同产地重楼中9种甾体皂甙类化合物进行了分析,结果提示不同产地重楼中各成分含量差异较大。

Abstract: A reversed phase HPLC method for the separalion and determination of nine steroidal saponins in Rhizoma paridis is deseribed The column WaS Symnletry C₈，mobile phase Was aeelonilrile—water(42：58)，UV detection WaS performed at 203 nm The calibration curves showed good linlearity over lhe range of 0.8～12.0 ug，γ=0.9996～0.9999．the recoveries were 97.1％～100.2％for the nine steroidal saponins The method is simple，fast，sensitive and accuraie and has been applied to the analysis of Rhizoma paridis. The results showed that the eontenis of the nine steroidal saponins in Rhizoma paridis were different with different sourees.