异丹叶大黄素和白藜芦醇对小鼠腹腔巨噬细胞白细胞介素6 mRNA表达的影响

EFFECTS OF ISORHAPOTIGENIN AND RESVERATROL ON INTERLEUKIN 6 mRNA EXPRESSION FROM STIMULATED PERITONEAL MACROPHAGES OF MICE

摘要: 目的：观察异丹叶大黄素和白藜芦醇对分别由炎性三肽(fMLP)和钙离子载体A₂₃₁₈₇(A₂₃₁₈₇)诱导的小鼠腹腔巨噬细胞白细胞介素6(interleukin 6, IL-6) mRNA表达水平的影响。方法：用半定量mRNA的RT-PCR。结果：异丹叶大黄素和白藜芦醇在2×10^-6和2×10^-5 mol.L^-1浓度下，均能抑制fMLP和A₂₃₁₈₇诱导的小鼠腹腔巨噬细胞IL-6 mRNA的表达。结论：该2个化合物抑制IL-6活性的作用机制之一是抑制IL-6 mRNA表达。

Abstract: AIM: Effects of isorhapotigenin and resveratrol on interleukin 6 mRNA expression from mouse peritoneal macrophages stimulated with fMLP and A₂₃₁₈₇ in succession were explored. METHODS: Interleukin 6 mRNA was semiquantified by RT-PCR. RESULTS: Isorhapotigenin and resveratrol, at the concentrations of 2×10^-6 and 2×10^-5 mol.L^-1, were shown to inhibit interleukin 6 mRNA expression from mouse peritoneal macrophages stimulated with fMLP or A₂₃₁₈₇ in succession. CONCLUSION: Inhibition of interleukin 6 mRNA expression may be the mechanism of the interleukin 6 biosynthetic inhibition of isorhapotigenin and resveratrol.