Abstract: Arctium lappaol Linne is a kind of traditional Chinese medicine used as anticancerand antinephritis agent.Twelve lignans have been isolated from its seeds. A reversed phase HPLC method for the separation and determination of the five lignans:arctiin(Ⅰ) ,lappaol A(Ⅱ),lappaol F(Ⅲ),arctigenin(Ⅳ),neoare B(Ⅴ)in the seeds of Arctium lappaolLinne is described,The column was 25 cm × 6 mm ID, packed with ODS(10μm).The columntemperature was 28℃. The mobile phase was a mixture of methanol-water-acetonitrile-tetrahydrofuran(57 :49 :11 :1).The flow rate was 1.0～1.5 min^-1. Diazepam was used asan internal standard,UV detection was performed at 220 nm. The relative standard deviation of the method was 2.16～2.96%. The calibration curves showedgood linearity over the range of 0.029～0.242μg,r=0.9993～0.9999 and the detection limitswere 3.38～4.20 ng.The recoveries were 96.14～104. 99%for the five lignans. The method is simple,fast, sensitive and accurate and has been applied to the analysis of Arctiumlappaol Linne of different sources.It can also be used for the quality control of Arctium preparations forclinical uses.