真菌诱导子对滇重楼中次生代谢产物甾体皂苷的影响

Effects of fungal elicitors on the secondary metabolite steroidal saponin in Paris polyphylla var. yunnanensis

摘要:
为了研究比较接种和不接种丛枝菌根 (AM) 真菌对滇重楼根茎中甾体皂苷类成分的影响, 通过室温盆栽接种试验, 采用高效液相色谱法测定并比较收获滇重楼根茎中甾体皂苷类成分的差异。结果显示, 不同AM真菌对滇重楼根茎中次生代谢产物甾体皂苷的影响存在差异。AM真菌诱导子处理后, 没有改变滇重楼根茎的化学背景, 但能部分提高其根茎中化学成分的含量。实验结果表明, AM真菌与滇重楼之间具有选择性, 根内球囊霉是最适宜滇重楼接种的优良菌种。

Abstract:
To compare the effects of inoculated or non-inoculated with arbuscular mycorrhizal (AM) fungi on the steroidal saponin component in root of Paris polyphylla var. yunnanensis. By pot experiments, steroid saponin component in root of P. polyphylla var. yunnanensis was determined and compared by HPLC. The esults showed there was difference in the effects of different AM fungal on the secondary metabolite steroid saponin in P. polyphylla var. yunnanensis. After elicitors treatment, AM fungal did not change the chemical backgrounds of P. polyphylla var. yunnanensis, but can improve partly the content of chemical compositions in roots. In conclusion, there was selectivity between AM fungal and P. polyphylla var. yunnanensis. Glomus intraradices was the most appropriate strain for inoculation P. polyphylla var. yunnanensis.