库仑滴定法测定血清胆固醇的含量

DETERMINATION OF CHOLESTEROL IN HUMAN SERUM BY COULOMETRIC TITRATION

摘要: 目的：研究库仑滴定法测定血清胆固醇含量的分析方法。方法：以1 mol.L^-1 KBr—冰醋酸(1∶3)为电解液，在阳极电生溴与胆固醇发生加成反应测定胆固醇含量。结果：胆固醇与溴反应，其反应电子数n为2。胆固醇的加入量为0.115～0.459 mg时，其回收率为93.9%～105%，平均回收率为100.3%，方法的RSD<1.5%。应用本方法对人血清样品中胆固醇含量进行了测定，并与酶法测定结果进行了对比。结论：本法简便、快速、准确，可用于血清胆固醇含量的测定。

Abstract: AIM: To study the determination method of cholesterol in human serum by Coulometric titration. METHODS: The titration was carried out in an electrolytic solution containing 1 mol.L^-1 potassium bromide and glacial acetic acid (1∶3). Bromine was electrogenerated at the anode and cholesterol reacted with bromine with a value of reactional electrons (n) as 2. RESULTS: The ranges of recovery for 0.115 mg to 0.459 mg of cholesterol were from 93.9% to 105%, and the average recovery was 100.3%. The relative standard deviation in all applied analysis was less than 1.5%. The accuracy of the method was also assessed in comparison with the results obtained by the enzymatic method used clinically. CONCLUSION: The method proposed is simple, rapid and accurate, and is suitable for the determination of cholesterol in human serum samples.